Jumat, 05 Oktober 2012

Pneumococcus


 Pewarnaan gram:
Coccus, seperti lanset 2-2 

             Pewarnaan Kapsul (Burry-Gins)


         Agar Darah: Jernih, halus, Bulat, hemodigesti
         TSB: Keruh
Uji Sensitivity (Optochin)



Cara:
Buat suspensi kuman dengan NaCl fis ~stdr Mc Farland 0,5.
Masukkan lidi kapas steril, oleskan pada Muller Hinton Agar .
Letakkan Bacitrasin sebnyak 4 Buah.
Eramkan 24 jam 37°C.

Streptococcus pneumonia sensitive terhadap optochin




Kelarutan empedu:

1 cc Nacl+koloni sampai kekeruhan~Mc Farland.
+ 3-4 tetes larutan empedu(Na desoxycholate 10%)
Inkubasi 15 menit 37’C.
Hasil positifàlarutan jernih
Karena Pneumococcus berkapsul maka polisakarida dalam kapsul akan larut dalam empedu.


Uji Inulin:
         Biakan ditanam pada gula Inulin inkubasi 37’C 24 jam. Hasil     positif pink->kuning.



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